| 表达新城疫病毒F48E8株融合蛋白基因的重组鸡痘病毒及其免？… |
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| 引用本文： | 吴艳涛 彭大新. 表达新城疫病毒F48E8株融合蛋白基因的重组鸡痘病毒及其免？…[J]. 生物工程学报, 2000, 16(5): 591-594 |
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| 作者姓名： | 吴艳涛 彭大新 |
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| 作者单位： | 扬州大学农业部畜牧禽传染病学重点开放实验室,扬州 |
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| 基金项目： | 江苏省九·五攻关项目(BE96454)、江苏省新的农业科技项目(BG98501-13)。 |
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| 摘 要： | 将将城疫病毒（ＮＤＶ）Ｆ４８Ｅ８株融合蛋白基因导入鸡痘病毒（ＦＰＶ）插入载体ｐＥＧＦ１１７５－１的Ｐ７．５启动子下游，得到转移载体ｐＦＧ１１７５－１重组质粒。采用脂质体转染技术，将该质粒转染ＦＰＶ２８２Ｅ株感染的鸡胚成纤维细胞（ＣＥＦ）。，经过多次蓝斑筛选纯化，获稳定的重组病毒ｒＦＰＶ－ＮＤＦ。间接免疫荧光试验表明，ｒＦＰＶ－ＮＤＦ感染的ＣＥＦ中表达了ＮＤＶ的融合蛋白。用ｒＦＰＶ－ＮＤＦ免疫的ＳＦ
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| 关 键 词： | 重组鸡痘病毒 新城疫 融合蛋白 免疫力 |
| 文章编号： | 1000-3061-(2000)05-0591-04 |
| 修稿时间： | 2000-01-03 |
A Recombinant Fowlpox Virus Expressing the Fusion Protein of Newcastle Disease Virus Strain F48E8 and Its Protective Efficacy |
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| WU Yan-Tao,PENG Da-Xin,LIU Xiu-Fan,LIU Wei-Zhong,ZHANG Ru-Kuan. A Recombinant Fowlpox Virus Expressing the Fusion Protein of Newcastle Disease Virus Strain F48E8 and Its Protective Efficacy[J]. Chinese journal of biotechnology, 2000, 16(5): 591-594 |
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| Authors: | WU Yan-Tao PENG Da-Xin LIU Xiu-Fan LIU Wei-Zhong ZHANG Ru-Kuan |
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| Affiliation: | WU Yan-Tao,PENG Da-Xin,LIU Xiu-Fan,LIU Wei-Zhong,ZHANG Ru-Kuan;(Ministry Key Laboratory of Aniomal Infectious Diseases, Yangzhou University, Yangzhou 225009) |
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| Abstract: | The transfer vector pFGF1175-1 was constructed by insertion the fusion protein gene of Newcastle disease virus (NDV) F48E8 strain into the insertion vector pFG1175-1, downstream of P7.5 promotor, and then transfected chicken embryo fibroblast (CEF) cell cultures which had been infected with fowlpox viras (FPV) Chinese vaccine strain 282E4 for 3-4 hours. Recombinant FPV with blue plaques were selected and purified in CEF cell culture laid agar containing X-gal. The recombinant FPV named rFPV-NDF was confirmed expressing NDV fusion protein by indirect immunofluorescence assay, and could protect chickens against virulent NDV challenge, The protective rate was 96.7%. |
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| Keywords: | Newcastle disease virus fusion protein recombinant fowlpox virus |
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