Changes in Saccharomyces cerevisiae ascospore proteins during early germination |
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| Authors: | Thomas P. West Robert L. Armstrong Paul T. Magee |
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| Affiliation: | Department of Microbiology and Public Health, Michigan State University, East Lansing, MI 48824, U.S.A. |
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| Abstract: | Abstract Proteins present in Saccharomyces cerevisiae ascospores and in germinating ascospores were compared using two-dimensional polyacrylamide gel electrophoresis. Yeast ascospores incorporated labeled methionine efficiently facilitating the electrophoretic analysis. Proteins synthesized in the yeast ascospores differed significantly from those proteins found 15 min after the initiation of germination in the ascospores. An immediate transition from ascospore proteins to proteins required for ascospore germination appears likely. |
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| Keywords: | Two-dimensional gels methionine incorporation protein modification protein synthesis |
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