Particle Counter Determination of Bacterial Biomass in Seawater |
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Authors: | Kazuhiro Kogure and Isao Koike |
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Abstract: | The applicability of the Elzone particle counter to the determination of marine bacterial biomass was investigated. The biomass of bacterial pure cultures and a mixed natural population were followed by using the particle counter, a CHN analyzer, and an ATP analyzer. The particle counter showed the precise size distribution of number and volume of submicron-size particles in seawater. For the pure cultured bacterial strains, the conversion factor from volume to carbon is 0.209 mg of C per mm3, and for natural bacterial cells of >0.6 μm in diameter, it is 0.184 mg of C per mm3. It is recommended that 0.2 be used as the conversion factor for both pure cultured marine bacterial cells and natural bacteria from coastal and near-shore marine environments. |
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