The temperature dependence of the fluorescence decay of low-density lipoproteins |
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Affiliation: | European Molecular Biology Laboratory, Postfach 10.2209, D-6900 HeidelbergF.R.G. |
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Abstract: | The decay of the intrinsic tryptophan fluorescence of low-density lipoproteins (LDL) is measured using a picosecond laser system with an excitation wavelength of 295 nm. The emission wavelengths were observed at 320, 340 and 360 nm. The measurements were performed in the temperature range 10–45°C. The fluorescence decay data are analysed in terms of: (i) three exponentials; (ii) a continuous distribution of exponentials; and (iii) the average decay time. The results indicate that the tryptophans are in a non-polar environment and that the fluorescence is not affected by phase transitions found to occur in the lipid core of the LDL molecule. |
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