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Presence of translatable mRNA in diapause eggs of Bombyx mori
Affiliation:1. Laboratory of Sericulture, Faculty of Agriculture, Kyushu University, Fukuoka 812 Japan;2. Laboratory of Food and Nutrition, Seinan-Jogakuin Junior College, Kokura-Kitaku, Kitakyushu 803, Japan;1. Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;2. Cancer Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;3. School of Human and Social Sciences, University of West London, London, UK;1. Division of Rheumatology, Inflammation and Immunity, Brigham and Women’s Hospital, Boston, MA, USA;2. Department of Medicine, Harvard Medical School, Boston, MA, USA;3. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia;4. Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia;5. Harvard Initiative for RNA Medicine, Boston, Massachusetts, USA;1. Department of Biochemistry, University of Washington, Seattle, WA 98195, USA;2. Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98109, USA;3. Paul G. Allen School of Computer Science & Engineering, University of Washington, Seattle, WA 98195, USA;4. Department of Comparative Medicine, University of Washington, Seattle, WA 98195, USA;5. Department of Molecular and Cellular Biology, University of Washington, Seattle, WA 98109, USA;6. Department of Medicine, Division of Hematology, University of Washington, Seattle, WA 98195, USA;7. Department of Bioengineering, University of Washington, Seattle, WA 98195, USA;8. West Coast Metabolomics Center, University of California, Davis, Davis, CA 95616, USA;9. Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA;10. Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA;1. Institute of Zoology, Zoological Society of London, London, UK;2. University College London, Gower Street, London WC1E 6BT, UK;3. School of Biological Sciences, Monash University, Victoria 3800, Australia;4. BirdLife Australia, 60 Leicester Street, Carlton, Victoria 3053, Australia;5. Wildlife Ecology Group, Institute of Natural Resources, Massey University, Palmerston North, New Zealand;6. Department of Zoology, University of Otago, Dunedin, New Zealand
Abstract:Total RNA extracted from diapause eggs of Bombyx mori was found to be translatable in a cell-free heterologous protein synthesis system derived from wheat germ or rabbit reticulocyte lysate. This supports the idea that active mRNAs are contained in the polysomes of diapause eggs.
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