Oxygraphic assay of 3,4-dihydroxyphenylalanine decarboxylase activity by coupled reaction with free and immobilized serum amine oxidase |
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| Affiliation: | 1. Istituti di Chimica Biologica e Biochimica Applicata, Facoltà di Farmacia, Università di Roma ‘La Sapienza,’ Rome, Italy;2. Centro di Biologia Moleculare, CNR, Rome, Italy;3. Istituto di Patologia Generale, Università di Padova, Padua, Italy;1. Department of Neurosurgery, Huashan Hospital Affiliated to Fudan University, 12 Wulongmuqi Middle Road, Shanghai, 200040, PR China;2. Department of Biochemistry, University of California, San Diego, USA;3. Department of General Medicine, Yangpu Hospital, Tongji University School of Medicine, 450 Tenyue Road, Shanghai 200090, PR China;4. Teaching and Research Group of Classic Documents of Traditional Chinese Medicine, Changhai Hospital of Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, PR China;1. Orthopedics Department, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Pudong, Shanghai, 201399, China;2. Orthopedics Department, Zhabei Center Hospital of JingAn District of Shanghai, JingAn, Shanghai, 200070, China;3. Tuberculosis Clinical Research Center, Shanghai Pulmonary Hospital Affiliated to Tongji University, Shanghai, 200070, China |
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| Abstract: | An oxygraphic method for the assay of 3,4-dihydroxyphenylalanine (Dopa) decarboxylase activity which makes use of the coupled reactions of Dopa decarboxylase with serum amine oxidase is presented. Both free and immobilized amine oxidases were utilized. The assay is simple, rapid, and allows a continuous monitoring of the reaction. The kinetic parameters for Dopa decarboxylase obtained with the coupled assay do not significantly differ from those obtained by standard methods. |
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