(1) Institute of Animal Physiology, Slovak Academy of Sciences, Soltesovej 4-6, 04001 Kosice, Slovak Republic;(2) Faculty of Science, Institute of Chemistry, P. J. Safarik University, Moyzesova 11, 04001 Kosice, Slovak Republic
Abstract:
Two GATC specific methylases together with Sau3AI isoschizomeric restriction endonuclease were partially characterized in
Mitsuokella multiacida 46/5. This is the first report on the presence of solitary Dam methyltransferase alongside GATC specific restriction-modification
system resulting in the unusual two-fold methylation of the GATC motifs.