Translation of polyuridylic acid in lysed mitochondria |
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| Authors: | S Alziari F Berthier R Debise S Touraille R Durand |
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| Institution: | 1. Shriram Institute for Industrial research, New Delhi, India;2. Animal Resources Development Department, Gurkhabusti, Tripura, India;3. Consultancy for Environmental & Human Toxicology and Risk Assessment (Fr), India Office, New Delhi, India;4. Department of Chemistry, University Institute of Science, Chandigarh University, Gharuan, Mohali, Punjab, India;1. Resident, Department of Prosthodontics, School of Medicine, Ewha Womans University, Seoul, Republic of Korea;2. Assistant Professor, Department of Restorative Dentistry and Biomaterials Sciences, Harvard School of Dental Medicine, Boston, Mass;3. Professor and Chairman, Department of Prosthodontics, School of Medicine, Ewha Womans University, Republic of Korea;4. Assistant Professor, Department of Prosthodontics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea;2. Department of Animal Science, University of California, Davis 95616;3. Department of Animal Sciences, The Ohio State University, Columbus 43210;4. School of Environmental Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada;1. University of Novi Sad, Faculty of Sciences, Department of Chemistry, Biochemistry and Environmental Protection, Trg D. Obradovića 3, 21000 Novi Sad, Serbia;2. Institute of Chemistry, Technology and Metallurgy, Department of Catalysis and Chemical Engineering, University of Belgrade, Njegoševa 12, 11000 Belgrade, Serbia;3. Vinča Institute of Nuclear Sciences, University of Belgrade, Mike Petrovića Alasa 12-14, 11001 Belgrade, Serbia;1. Department of Economics, Monash University, Malaysia;2. Universal College Lanka, Sri Lanka;3. Faculty of Economics and Administration, University of Malaya, Malaysia;4. School of Economics, Reading University, UK;5. Centre on Skills, Knowledge and Organisational Performance, Oxford University, UK;6. IZA Institute of Labor Economics, Bonn, Germany;7. Global Development Institute, University of Manchester, UK |
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| Abstract: | After osmotic shock with 50 mM Tricine buffer (pH 7.9), isolated mitochondria from D. Melanogaster embryos are treated with a low concentration of Triton X-100 (25 micrograms/mg of protein). The lysed mitochondria are still capable of RNA and protein synthesis. While incorporation of labeled precursor is often higher in lysed than in intact mitochondria, neosynthesized proteins exhibit similar electrophoretic patterns. Studies of labeled precursor incorporation in the presence of various effectors indicate a better accessibility to the translation machinery in lysed mitochondria than in intact mitochondria. Such a system has proven capable of translating an exogenous synthetic mRNA, i.e., poly (U). |
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