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用TnV分离粘细菌发育回复突变株
引用本文:毛晓华,汪道涌,李新荣.用TnV分离粘细菌发育回复突变株[J].微生物学报,2001,41(2):241-243.
作者姓名:毛晓华  汪道涌  李新荣
作者单位:东南大学医学院生化教研室,南京,210009
基金项目:国家自然科学基金资助项目(39800079)
摘    要:fruA∷TcΩ5 is a development deficient strain of M.Xamthus.Transposon TnV was used to randomly mutagenize various sites of fruA ∷TcΩ5 chromosome.Fruiting body formation was restored in one TnV insertion mutant,designated XM1206.The TnV\|inserted DNA fragment from XM1206 chromosome was cloned,which may be served as a probe to isolate the corresponding allele from wild\|type strain.

关 键 词:粘细菌    发育    回复突变
文章编号:0001-6209(2001)02-0241-03
修稿时间:2000年4月28日

ISOLATION OF A DEVELOPMENT REVERTANT OF MYXOCOCUS XAMTHUS
X Mao,D Wang,X Li.ISOLATION OF A DEVELOPMENT REVERTANT OF MYXOCOCUS XAMTHUS[J].Acta Microbiologica Sinica,2001,41(2):241-243.
Authors:X Mao  D Wang  X Li
Institution:Department of Biochemistry, Southeast University Medical College, Nanjing 210009, China.
Abstract:fruA::Tc omega 5 is a development deficient strain of M. Xamthus. Transposon TnV was used to randomly mutagenize various sites of fruA::Tc omega 5 chromosome. Fruiting body formation was restored in one TnV insertion mutant, designated XM1206. The TnV-inserted DNA fragment from XM1206 chromosome was cloned, which may be served as a probe to isolate the corresponding allele from wild-type strain.
Keywords:Myxbacteria  Development  Revertant mutant
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