Occurrence of Two Types of Ca2+-Dependent Protein Kinases in the Cytosol Fraction of the Brain |
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| Authors: | E. Miyamoto K. Fukunaga K. Matsui Y. Iwasa |
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| Affiliation: | Department of Pharmacology, Kumamoto University Medical School, Kumamoto, Japan |
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| Abstract: | Abstract— Two types of Ca2+-dependent protein kinases were demonstrated and partially purified from the cytosol fraction of rat brain by DEAE-cellulose, Sephadex G-200, and calmodulin-affinity column chromatography, using endogenous proteins and chicken gizzard myosin light chains as substrates. The molecular weights of the enzymes were 88,000 (peak I) and 120,000 (peak II) on gel filtration. Peak I had no affinity for calmodulin, whereas peak II had a high affinity for it, with a K a value of 16.7 n m . The K a values of peaks I and II for Ca2+ were 2.4 and 1.6 μ m , respectively. |
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