Isolation of a spontaneously fusing BC3H1 muscle cell line: fusion alters the response to serum stimulation |
| |
Authors: | Thomas Steenstrup Kevin Hannon |
| |
Institution: | (1) Department of Basic Medical Sciences, School of Veterinary Medicine, Purdue University, 47907 West Lafayette, Indiana |
| |
Abstract: | Summary Differentiation of skeletal muscle cells involves two distinct events: exit from the cell cycle and expression of musclespecific
contractile genes and formation of multinucleated myocytes. Although many studies have shown that growth factors regulate
the initial step of differentiation, little is known about regulation of fusion. BC3H1 cells are a skeletal muscle cell line
characterized by a nonfusing phenotype and an ability to dedifferentiate. When subjected to serum or growth factors, differentiated
BC3H1 cells lose muscle-specific gene expression and re-enter the cell cycle. In this study, we describe a spontaneously fusing
clone of BC3H1 cells. We demonstrate that this fusion capability is not due to altered muscle regulatory factor or adhesion
molecule expression. Furthermore, we show that fusion inhibits dedifferentiation. Multinucleated BC3H1 cells do not lose myosin
expression, nor do they re-enter the cell cycle. Fused BC3H1 cells react to serum stimulation with a hypertrophic response.
Our results suggest that the state of differentiation, mono- or multinucleated, is essential to how myocytes react to growth
stimulation and may provide a mechanism for how differentiation, fusion, and hypertrophy are regulated in vivo. |
| |
Keywords: | BC3H1 cells muscle differentiation myoblast fusion growth factor MyoD |
本文献已被 PubMed SpringerLink 等数据库收录! |
|