金沙江干热河谷蕃麻皂素资源的开发研究

借助已有的酸水解/醇提取的方法,分析了来自四川攀枝花、成都、海南西部、南非中部4个不同生境地域的共10个蕃麻鲜叶片样品,以及来自云南楚雄元谋、保山施甸、丽江永胜、四川攀枝花4个典型的金沙江干热河谷地域的15个蕃麻麻膏(干料,含水量15%~20%)样品,得到了其甾体总甙元组分、含量等基础数据资料。并在此基础上,重点开展了对蕃麻皂素的提取、分离、精制工作,建立了合适的工艺技术路线,乙酰蕃麻皂素产率4.7%。结果表明:金沙江干热河谷地域很适合蕃麻生长,而且生源积累优势组分蕃麻皂甙元,利于后加工,很有希望成为我国甾体药业新的原料基地。蕃麻作为一种种植业发展潜力巨大。

Study on the Resource of Hecogenin in Jinsa River Dry-hot Valley

Ten samples of Agave americana L.leaves gathered from four different climes namely Sichuan Panzhihua,Chengdu,western of Hainan,midland of South Africa and 15 crude extracted samples of Agave americana L.(moisture content 15%-20%) gathered from 4 typicale zones in Jinsa River dry-hot Valley namely Yunnan yuanmou chuxiong,baoshan shidian,lijiang yongsheng,Sichuan Panzhihua were examined in virtue of acid hydrolysis and extracted with ethanol.Some of useful basic data of steroidal sapogenins has been obtained.Moreover,on the base of the work,we put emphasis on the work of extraction,separation and refinement of the hecogenin.Therefore,an appropriate process was already developed with the hecogenin acetate yield 4.7%.The results shown that Agave americana L.adapted to growth in Jinsa River dry-hot Valley and accumulated high content of Hecogenin as a main component from a total of steroidal sapogenins so that it is benefit to next steps of bioprocessing and chemical processes.Agave americana L.has great potential as a planting in Jinsa River dry-hot Valley and it is expected that the region Jinsa River dry-hot Valley would become a new raw material base for industrial steroidal drugs in China.