Expression and secretion of goat alpha-lactalbumin as an active protein in Saccharomyces cerevisiae |
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| Authors: | S Takeda E Tamaki K Miura I Kumagai |
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| Institution: | Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan. |
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| Abstract: | An expression plasmid for goat alpha-lactalbumin in Saccharomyces cerevisiae, pSKA100, was constructed into a shuttle vector, pYG100, by inserting cDNA which encodes goat pre-alpha-lactalbumin and two-thirds of the 3'-non-coding region. The goat alpha-lactalbumin was expressed under the yeast glyceraldehyde 3-phosphate dehydrogenase (GPD) promoter and terminator of pYG100 and secreted in the growth medium for yeast as a precise mature protein, possessing specific activity essentially the same as that of authentic goat alpha-lactalbumin in lactose synthesis. |
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