Micropropagation of<Emphasis Type="Italic">Schizandra chinensis</Emphasis> BAILLON using glucose from cotyledonary nodes |
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Authors: | Min?Hee?Hong Ok?Tae?Kim Jae?In?Park Email author" target="_blank">Baik?HwangEmail author |
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Institution: | (1) Department of Biology, Chonnam National University, 500-757 Gwangju, Korea;(2) School of Forest Resources, Chungbuk National University, 361-763 Chungbuk, Korea |
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Abstract: | A protocol for the micropropagation ofSchizandra chinensis has been developed using regenerated shoots from axillary bud explants. In preparing to do so, we found that seed type (i.e.,
mature vs. pre-mature) significantly influenced the rate of germination. The Woody Plant (WP) medium proved to be superior
to the Murashige and Skoog (MS) medium for germination purposes. Multiple shoots were induced from cotyledonary nodes of axenic
seedlings on WP media containing 6-benzylaminopurine (BA) alone or in combination with 1-napthaleneacetic acid (NAA). High
frequencies of shoot proliferation and the greatest number of shoots per explant (11.6) were observed with the use of 1.0
mg L-1 BA. We also established a culture method for proliferating shoots by repeatedly subculturing the original cotyledonary nodes
on a shoot multiplication medium each time newly formed shoots were harvested. To induce root formation, glucose was supplied
as a carbon-source substitution for sucrose. The best rooting rate was obtained from a WP medium supplemented with 3% glucose
and 0.5 mg L-1 NAA. Following transplantation in the field, 82% of the plantlets survived. |
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Keywords: | cotyledonary node glucose micropropagation Schizandra chinensis |
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