鱼藤酮致帕金森大鼠的脑黑质中凋亡相关蛋白Bcl-2、Bax表达改变

目的研究鱼藤酮致帕金森病（PD）大鼠中脑黑质凋亡相关蛋白Bcl-2、Bax表达的改变。方法Wistar大鼠每日颈背部皮下注射鱼藤酮2mg（kg·d）（3～6周）造模,依据所建立的评分体系记录动物行为变化,在行为学有记分并停止给鱼藤酮4、10d时,中脑黑质病理切片免疫组化染色比较黑质区域Bcl-2、Bax的表达。结果在有行为学记分4d时,记4分和8分的大鼠中脑黑质Bcl-2表达均显著减少;所有PD大鼠中脑黑质Bax表达均显著增加;Bcl-2/Bax比率均显著减少;有记分4d时,行为学记分与Bcl-2/Bax比值成负相关性。结论细胞凋亡参与了鱼藤酮帕金森模型大鼠黑质多巴胺神经细胞的损伤。

Changes of Expression of Apoptosis-Related Proteins Bcl-2 and Bax in Parkinson's Disease Rat Induced by Rotenon

Objective To study the changes of expression of apoptosis-related proteins Bcl-2 and Bax in the midbrain substantia nigra in Parkinson＇s disease rat induced by rotenone.Method Rotenone [2 mg/（kg·d）] was injected subcutaneously to the back of Wistar rats for 3～6 weeks to establish a rat model of Parkinson＇s disease.To observe and record the scores of rats according to the behavioral score system established in this laboratory.Immunohistochemical staining was used to compare the Bcl-2 and Bax expression in the mesencephalon substantia nigra of rats on the fourth and tenth day after behavior marking.Result On the fourth day of behavior marking,the Bcl-2 expression of the midbrain substantia nigra was reduced significantly in the rats with marks 4 and 8.The Bax expression was increased remarkably in all groups.The Bcl-2/Bax ratio was reduced.On the fourth day of marking,there was a negative correlation between the behavior marks and Bcl-2/Bax ratio.Conclusion Apoptosis participates in the impairment of dopaminergic neurons in the substantia nigra in PD rats induced by rotenone.