Collagen XVIII and corneal reinnervation following keratectomy |
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Authors: | Sakimoto Tohru Kim Tae-Im Ellenberg David Fukai Naomi Jain Sandeep Azar Dimitri T Chang Jin-Hong |
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Affiliation: | Schepens Eye Research Institute, Harvard University, Boston, MA, USA. |
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Abstract: | The significance of collagen XVIII in the regulation of corneal reinnervation remains largely unknown. We used whole-mount immunoconfocal microscopy to localize collagen XVIII to the nerve basement membrane of wild-type (WT) mouse corneas. Transmission electron microscopy showed corneal nerve disorganization in collagen XVIII knockout mice (col18a1(-/-)). Antibody 2H3-specific neurofilament colocalized with collagens XVIII and IV and laminin-2 in WT mouse corneas, but did not colocalize with collagen IV and laminin-2 in col18a1(-/-) mouse corneas. Following keratectomy, col18a1(-/-) mice displayed decreased corneal neurite extension compared to WT mice. Our data indicate that collagen XVIII may play an important role in corneal reinnervation after wounding. |
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