Cloning and structure of a mouse interleukin-2 chromosomal gene |
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Authors: | W Degrave G Simons R Devos G Plaetinck E Remaut J Tavernier W Fiers |
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Institution: | (1) Laboratory of Molecular Biology, State University of Ghent, Ledeganckstraat 35, B-9000 Ghent, Belgium;(2) Present address: N.I.Z.O., Kernhemseweg 2, Postbus 20, 6710 BA Ede, The Netherlands |
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Abstract: | Using non-stringent hybridization with a human interleukin-2 cDNA probe, we have isolated recombinant phages from a mouse genomic DNA library cloned in the EMBL3 phage. The sequence and organization of the mouse interleukin-2 (IL-2) gene was determined. By comparison with the human IL-2 sequence, three introns can be identified with lengths of 99, ±2 400, and ±1 900 base pairs, respectively. The mouse IL-2 gene codes for a polypeptide of 169 amino acids and contains a putative signal peptide of 20 amino acids. The homology to the human interleukin-2 is 72% at the nucleotide level in the coding part and 65% at the amino acid level. An extraordinary sequence, consisting of 12 consective CAG codons coding for glutamine, is found in the first exon. |
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