Effect of culture medium composition on inhibition of growth ofNeisseria gonorrhoeae by lactobacilli |
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Authors: | A Morin S A Saheb J G Bisaillon R Beaudet M Sylvestre |
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Institution: | (1) Centre de Recherche en Bactériologie, Institut Armand-Frappier, Université du Québec, Laval-des-Rapides, C. P. 100, H7N 4Z3 Ville de Laval, Québec, Canada |
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Abstract: | The role of genital microorganisms in resistance to gonococcal infection is usually based on their in vitro inhibition of
gonococcal growth. Three different culture media (GC, DSA, and MRS) were evaluated for their ability to support the growth
of 23 lactobacilli strains and the detection of the antigonococcal activity of these bacteria. The MRS medium was the most
suitable medium for the growth of lactobacilli since it favored a good growth of all the lactobacilli strains tested, but
it was inhibitory toNeisseria gonorrhoeae. Decreasing the concentration of Tween 80, ammonium citrate, and sodium acetate to one-tenth of their original concentrations
yielded a modified MRS medium which still supported good growth of the lactobacilli and was no longer inhibitory to the gonococci.
While GC medium did not allow any detection of the production of antigonococcal activity by the lactobacilli, both modified
MRS and DSA media allowed the detection of this activity by the agar overlay technique. The use of modified MRS medium is
recommended since it is less selective than DSA medium for the growth of lactobacilli. |
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