Cotranslational fatty acylation of mucus glycoprotein. Addition of palmitic acid to peptidyl-tRNA occurs prior to peptide chain completion and its release |
| |
| Institution: | 1. Department of Pharmacy, BITS-Pilani Hyderabad Campus, Hyderabad, India;2. Centre for Molecular Biology, Central University of Jammu, Samba, India;1. Department of Energy & Mineral Resources Engineering, Sejong University, Seoul 05006, Republic of Korea;2. Urban Mine Department, Korea Institute of Geoscience and Mineral Resources, 124 Gwahang-no, Yuseong-gu, Daejeon, Republic of Korea;1. Department of Physics, University of Agriculture Faisalabad, 38040, Pakistan;2. Department of Chemistry, College of Science, King Khalid University Abha, 61413, Saudi Arabia;3. Department of Chemistry, University of Okara, 56300, Pakistan;1. Department of Pharmacology and Physiology, University of Montreal, Montreal, QC, Canada;2. Department of Biomedical Engineering, University of Manitoba, Winnipeg, MB, Canada;1. EaStCHEM, School of Chemistry, University of Edinburgh, Joseph Black Building, West Mains Road, Edinburgh, EH9 3FJ, UK;2. School of Engineering, Institute for Bioengineering, The University of Edinburgh, The King''s Buildings, Mayfield Road, Edinburgh EH9 3JL, UK;1. Department of Biotechnology, Stella Maris College (Autonomous), Affiliated to University of Madras, Chennai, Tamil Nadu, India;2. Department of Chemical Engineering, Sri Sivasubramaniya Nadar College of Engineering, Kalavakkam, 603110, Chennai, India;3. Centre of Excellence in Water Research (CEWAR), Sri Sivasubramaniya Nadar College of Engineering, Kalavakkam, 603110, Chennai, India |
| |
| Abstract: | - 1.1. The fatty acylation of mucus glycoprotein nascent peptides was investigated using 3H]palmitic acid and 35S]methionine-labeled peptidyl-tRNA of rat gastric mucous cells.
- 2.2. The mucus glycoprotein peptidyl-tRNA fraction was found to contain covalently bound palmitic acid in its complexes.
- 3.3. RNase digestion of the mucus glycoprotein peptidyl-tRNA released 3H]palmitic acid labeled peptides which, on SDS-polyacrylamide gel, separated into a multitude of bands ranging in size from 2000 to 60,000 Da.
- 4.4. The analyses of low molecular weight peptides revealed that palmitic acid was present in methionine-labeled peptides containing 30–43 amino acids and those of 18–25 amino acids or larger devoid of methionine, but was not identified in methionine-labeled peptides containing 10–15 amino acids.
- 5.5. The results indicate that the N-terminal fatty acylation of mucus glycoprotein nascent peptides is a cotranslational process which is occuring in an immediate vicinity of the signal peptide fragment.
|
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
