Localization of chicken muscle FBPase in cardiomyocyte nuclei |
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Authors: | Zmojdzian Monika Dziewulska-Szwajkowska Daria Dzugaj Andrzej |
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Affiliation: | Department of Animal Physiology, Zoological Institute, Wroclaw University, Cybulskiego 30, 50-205 Wroclaw, Poland. |
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Abstract: | Fructose 1,6-bisphosphatase (FBPase; EC 3.1.3.11) localization in cardiomyocyte nuclei has recently been investigated in mammals [FEBS Lett. 539 (2003) 51]. In this study, nuclear localization of FBPase in the cardiac muscle of the chicken was studied by immunohistochemistry and other methods. A result of the electron microscopic investigation was confirmed by immunoblotting analysis. Using MALDI Q-TOF mass spectrometry and Mascot program, the nuclear FBPase was identified as muscle chicken FBPase. FBPase activity in isolated cardiomyocyte nuclei was 5.9 mU/g. Nuclear FBPase was strongly inhibited by allosteric inhibitor AMP. I(0.5) for AMP was 0.16 microM and was the same as for the purified chicken muscle enzyme. |
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