The spatial pattern of DNA synthesis in Dictyostelium discoideum slugs

We have used [³H]DNA labelling and autoradiography to investigate the localisation of cells in S phase of the cell cycle during the aggregation and slug stages of Dictyostelium discoideum development. Our results indicate that S phase cells occur behind a sharp transverse boundary, which falls just below (or behind) the anterior tip of the aggregate or slug. PAS staining indicates that this is the boundary between the prestalk and prespore regions.