Membrane-associated Glycosyl Transferases in Cotyledons of Pisum sativum: Differential Effects of Magnesium and Manganese Ions |
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Authors: | Nagahashi J Mense R M Beevers L |
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Affiliation: | Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019. |
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Abstract: | In crude particulate fractions isolated from pea (Pisum sativum) cotyledons, the transfer of radioactivity from GDP-[14C]mannose to glycolipid appears to be preferentially stimulated by Mn2+ while the transfer to lipid-free residue is enhanced by Mg2+. In contrast, the transfer of radioactivity from UDP-N-acetyl-[14C]glucosamine to glycolipid shows preferential stimulation by Mg2+ while the transfer to lipid-free residue prefers Mn2+. These results are accounted for by the differential stimulation by Mg2+ and Mn2+ of glycosyl transferases associated with subcellular membranes which were separated by isopycnic sucrose density centrifugation. |
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