A new technique for preparing biominerals for atomic-force microscopy |
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| Authors: | L. M. Egerton-Warburton S. T. Huntington P. Mulvaney B. J. Griffin R. Wetherbee |
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| Affiliation: | (1) School of Botany, The University of Melbourne, 3052 Parkville, VIC, Australia;(2) Schools of Physics, The University of Melbourne, Parkville, Victoria;(3) Schools of Chemistry, The University of Melbourne, Parkville, Victoria;(4) Centre for Microscopy and Microanalysis, The University of Western Australia, Nedlands, Western Australia |
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| Abstract: | Summary We describe a methodology that enables the orienting and cleaving of biominerals to consistently yield low z-axis (height) surfaces suitable for high-resolution imaging by atomic-force microscopy. The methodology permits biominerals to be prepared without chemical fixation, resin embedding, or sectioning, and demonstrates a utility for a variety of silicified and calcified biominerals. |
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| Keywords: | Atomic-force microscopy Biominerals Cleaving |
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