Prevalence of complex vertebral malformation carriers among Polish Holstein-Friesian bulls |
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Authors: | Anna Ru?? Stanis?aw Kamiński |
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Institution: | University of Warmia and Mazury, Department of Animal Genetics, Oczapowskiego 5, 10-718 Olsztyn, Poland. |
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Abstract: | An increasing number of Holstein calf births exhibiting vertebral deformations has been detected in Denmark since 1999 by
a program monitoring the incidence of genetic diseases. Pedigree analysis demonstrated that the affected calves originated
from a family afflicted by an autosomally recessively inherited complex vertebral malformation (CVM) syndrome. To determine
the actual carrier frequency of the CVM-determining mutation in a population of Polish Holstein-Friesian (=Polish Black-and-White)
cattle, we examined 202 proven bulls (active in 2001–2005) used by 4 domestic artificial insemination companies and 403 unproven
bulls (under evaluation for breeding value). Out of the 605 bulls examined, 150 T/G heterozygotes were diagnosed, including
118 that were sons of known CVM carriers. Identification of a gene polymorphism in a bovine solute carrier family 35 member
3, termedSLC35A3, was conducted with the use of a new PCR-SSCP method (polymerase chain reaction - single stranded conformation polymorphism),
which - due to its ease of use and high reliability - can be applied in widespread screening programs aimed at reducing the
incidence of the CVM defect. |
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