Effects of mutations in <Emphasis Type="Italic">SGS1</Emphasis> and in genes functionally related to <Emphasis Type="Italic">SGS1</Emphasis> on inverted repeat-stimulated spontaneous unequal sister-chromatid exchange in yeast |
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Authors: | Dilip K Nag Steffany J Cavallo |
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Institution: | (1) Division of Molecular Medicine, Wadsworth Center, Center for Medical Sciences, 150 New Scotland Avenue, Albany, NY 12208, USA;(2) Department of Biomedical Sciences, School of Public Health, State University of New York, Albany, NY 12201, USA |
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Abstract: | Background The presence of inverted repeats (IRs) in DNA poses an obstacle to the normal progression of the DNA replication machinery,
because these sequences can form secondary structures ahead of the replication fork. A failure to process and to restart the
stalled replication machinery can lead to the loss of genome integrity. Consistently, IRs have been found to be associated
with a high level of genome rearrangements, including deletions, translocations, inversions, and a high rate of sister-chromatid
exchange (SCE). The RecQ helicase Sgs1, in Saccharomyces cerevisiae, is believed to act on stalled replication forks. To determine the role of Sgs1 when the replication machinery stalls at
the secondary structure, we measured the rates of IR-associated and non-IR-associated spontaneous unequal SCE events in the
sgs1 mutant, and in strains bearing mutations in genes that are functionally related to SGS1. |
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