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In search of suitable reference genes for gene expression studies of human renal cell carcinoma by real-time PCR

Authors:	Monika Jung Azizbek Ramankulov Jan Roigas Manfred Johannsen Martin Ringsdorf Glen Kristiansen Klaus Jung

Institution:	1.Department of Urology,Charité – Universit?tsmedizin Berlin, Campus Charité Mitte,Berlin,Germany;2.Institute of Pathology, Charité – Universit?tsmedizin Berlin, Campus Charité Mitte,Berlin,Germany;3.Republic Center of Urology,Bishkek,Kyrgyz Republic

Abstract:	Background Housekeeping genes are commonly used as endogenous reference genes for the relative quantification of target genes in gene expression studies. No conclusive systematic study comparing the suitability of different candidate reference genes in clear cell renal cell carcinoma has been published to date. To remedy this situation, 10 housekeeping genes for normalizing purposes of RT-PCR measurements already recommended in various studies were examined with regard to their usefulness as reference genes.

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