DDAH2启动子区-1150C/A多态性与中国汉族人群冠心病的遗传易感性研究

目的:研究DDAH2启动子区-1150 C/A rs805304多态性与中国汉族人群冠心病之间的相关性。方法:应用限制性片段长度多态性聚合酶链反应(PCR-RFLP)的分析方法,对381例冠心病患者和629例健康人群中DDAH2基因A-1150C rs805304多态进行基因分型。结果:病例组和对照组A-1150C rs805304位点基因型分布均符合Hardy-Wenberg平衡;两组间A-1150C位点基因型间无显著性的差异(P=0.34);病例组和对照组A-1150C位点的等位频率分布也无显著的差别,但是在冠心病病例中,A等位频率有低于C等位频率的趋势(P=0.069)。结论:DDAH2启动子区-1150 C/A rs805304多态性与中国汉族人群冠心病的发病不相关。

Functional Variant in DDAH2 Contributes to the Susceptibility of Coronary Heart Disease in a Chinese Population

Objective： To investigate the association of DDAH2 promoter -1150 C/A rs805304 polymorphisms with coronary heart disease susceptibility in a Chinese Han population by a case-control study. Methods： Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was used to genotype the SNP. We performed a case-control analysis including a total of 381cases and 629 healthy controls. Results： Genotype distribution of the DDAH2 rs805304 polymorphism in both groups were in Hardy-Weinberg equilibrium. Our pooled result indicated that there is no significant difference in the genotype distribution between the CAD cases and controls （P=0.34）. However, comparing with A allele frequency, the C allele frequency have a lower tendency in cases of coronary heart disease（P=0.069）. Conclusion： the polymorphism（rs805304） of DDAH2 is not associated with the CAD.