血源性乙型肝炎病毒、丙型肝炎病毒和艾滋病病毒核酸筛查系统的建立与应用

目的：建立同时实现乙型肝炎病毒（hepatitisBvirus，HBV）、丙型肝炎病毒（hepatitisCvirus，HCV）、艾滋病病毒（humanImmunodeficiencyVirus，HIV）检测的多重核酸筛查系统。方法：以HBV、HCV、HIV的保守序列为模板设计特异性引物和探针，通过核酸自动提取系统结合一步法RT-PCR技术平台，优化相关反应体系和条件，建立多重多色实时荧光PCR检测血源性传播病毒的核酸筛查系统。将该系统用于101387例血浆样本的筛查。结果：本研究建立的核酸筛查系统特异性好，HBV灵敏度可以达到20IU／ml，HCV灵敏度可以达到100IU／ml，HIV灵敏度可以达到50IU／mL。结论：本研究建立的核酸筛查系统具有高度自动化、高灵敏度、低成本等特点，适合我国血站系统推广使用。

Establishment and Application of Multi-color Nucleic Acid Screening System for the Detection of Blood Transmitted HBV,HCV and HIV

Objective： To establish a multiple nucleic acid screening system for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus in blood samples. Methods： Conservative sequences of HBV, HCV and H1V were used to design specific primers and probes, automatic nucleic acid extract system combined with one-step RT-PCR technology were used, the reaction system and conditions were optimized to establish multi-color and multiple real-time fluorescence PCR nucleic acid screening system for detection of blood transmitted viruses. The system was used in screening of 100,000 plasma samples of our hospital. Results： The established system was highly specific with the sensitivity of HBV detection of 20IU/ml, sensitivity of HCV detection of 100IU/ml and sensitivity of HIV detection of 50IU/ml. Conclusion： The nucleic acid screening system in this study is highly automatic, highly sensitive and inexpensive, it is applicable for nationwide generalization in blood centers to insure the clinical blood utilization safety.