低硒心肌损伤与钾离子通道蛋白的关系研究

目的：心肌上的离子通道蛋白与心肌损伤有很大的关系，本研究通过低硒喂养对C57BL／6小鼠心肌组织损伤的影响及其对钾通道蛋白的改变。方法：将实验小鼠分为4组：对照组，低硒30天组，低硒90天组和低硒180天组。采用低硒饲料（硒含量0．0045μg／g）喂养的方法建立低硒小鼠模型，对照组给予正常饲料（硒含量0．256μg/g），与低硒组同时喂养；硒含量的测定和HE染色方法观察心肌损伤情况，WesternBlotting方法检测其钾通道蛋白的表达。结果：低硒饲料喂养小鼠的心脏硒含量与正常饲料喂养的硒含量相比明显降低（P〈0．01）；并出现轻微的心肌损伤，钾通道蛋白的表达量在低硒30天组，低硒90天组和低硒180天组下调（P〈0．01）。结论：成功建立低硒小鼠模型，低硒能引起小鼠心肌损伤，这种改变可能有心脏的钾通道蛋白的表达水平有关。

Study of Relationship between Cardiomyocytes Injury Induced by Selenium-deficiency and Potassium Channel Protein

Objective： Many studies showed that ardiomyocytes injury had related to ion channel, to study the effects of melatonin on heart tissue in C57BL/6 mice caused by low selenium diet and discuss the possible mechanism. Methods： The mice were divided into four group, with three treatment groups fed with selenium-deficient diet （0.0045 ixg/g） for control group, 30 day low-selenium, 90 day low-selenium, 180 day low-selenium. Then selenium and heart tissues were examined in mice. Western Blotting was employed to identify the down-stream protein of potassium channel protein expression level. Results： Compared with the control group, the selenium levels of the 30 days, 90 days and 180 days selenium deficiency mice were reduce （P〈0.01） and there was cardiomyocytes injury. And the expression of potassium channel protein in 30 days, 90 days and 180 days selenium deficiency mice was also down-regulated （P〈0.05）. Conclusion： We established a selenium-deficient mice model successful. Low selenium can caused cardiomyocytes injury and these changes might be caused by the level of potassium channel protein.