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Determination of reference genes for circadian studies in different tissues and mouse strains |
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| Authors: | Rok Kosir Jure Acimovic Marko Golicnik Martina Perse Gregor Majdic Martina Fink Damjana Rozman |
| Institution: | (1) Center for Functional Genomics and Bio-Chips, Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Zaloska 4, SI-1000 Ljubljana, Slovenia;(2) Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, SI-1000 Ljubljana, Slovenia;(3) Medical Experimental Centre, Institute of Pathology, Faculty of Medicine, University of Ljubljana, Zaloska 4, SI-1000 Ljubljana, Slovenia;(4) Center for Animal Genomics, Veterinary Faculty, University of Ljubljana, Gerbiceva 60, SI-1000 Ljubljana, Slovenia;(5) Department of Haematology, University Medical Center Ljubljana, Zaloska cesta 7, SI-1000 Ljubljana, Slovenia |
| Abstract: | BackgroundCircadian rhythms have a profound effect on human health. Their disruption can lead to serious pathologies, such as cancer and obesity. Gene expression studies in these pathologies are often studied in different mouse strains by quantitative real time polymerase chain reaction (qPCR). Selection of reference genes is a crucial step of qPCR experiments. Recent studies show that reference gene stability can vary between species and tissues, but none has taken circadian experiments into consideration. |
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