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Identification and characterization of the intracellular poly-3-hydroxybutyrate depolymerase enzyme PhaZ of Sinorhizobium meliloti |
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| Authors: | Maria A Trainer David Capstick Alicja Zachertowska Kathy N Lam Scott RD Clark Trevor C Charles |
| Affiliation: | (1) Department of Biology, University of Waterloo, 200 University Ave W, N2L 3G1 Waterloo, ON, Canada;(2) Council of Canadian Academies, 180 Elgin St, Suite 1401, K2P 2K3 Ottawa, ON, Canada;(3) Department of Biology, McMaster University, 1280 Main St West, L8S 4K1 Hamilton, ON, Canada;(4) Department of Molecular Genetics, University of Toronto, 1 King's College Circle, M5S 1A8 Toronto, ON, Canada |
| Abstract: | BackgroundS. meliloti forms indeterminate nodules on the roots of its host plant alfalfa (Medicago sativa). Bacteroids of indeterminate nodules are terminally differentiated and, unlike their non-terminally differentiated counterparts in determinate nodules, do not accumulate large quantities of Poly-3-hydroxybutyrate (PHB) during symbiosis. PhaZ is in intracellular PHB depolymerase; it represents the first enzyme in the degradative arm of the PHB cycle in S. meliloti and is the only enzyme in this half of the PHB cycle that remains uncharacterized. |
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