In vitro development of early postimplantation rat embryos |
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Authors: | S K Buckley C E Steele D A New |
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Institution: | 1. Physiological Laboratory, Cambridge CB2 3EG, UK;2. Department of Surgery, Addenbrooke''s Hospital, Hills Road, Cambridge CB2 2QQ, England |
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Abstract: | Rat embryos explanted at or post coitum were cultured throughout the major stages of organogenesis in a system of rotating bottles containing heat-inactivated, immediately centrifuged (I.C.) serum. About 80% of the explants and 50% of the explants developed a blood circulation in the yolk sac; in these embryos, organogenesis and growth rates were similar to those of embryos in vivo. In cultures continued for 4 or 5 days, many of the embryos developed 30–40 somites. There was little difference in the subsequent development of embryos cultured in maternal serum or male serum during the egg-cylinder stage except for a possible decrease in the frequency of normal axial rotation in embryos from the male serum. Development in rotator bottles was much better than in watchglass cultures. |
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