Resolution of 1,2-epoxyhexane by Rhodotorula glutinis using a two-phase membrane bioreactor |
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| Authors: | W J Choi C Y Choi J A M De Bont C A G M Weijers |
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| Institution: | (1) Division of Industrial Microbiology, Department of Food Technology and Nutritional Sciences, Wageningen Agricultural University, PO Box 8129, 6700 EV Wageningen, The Netherlands e-mail: carel.weijers@imb.ftns.wau.nl Tel.: +31-317-483595 Fax: +31-317-484978, NL;(2) Department of Chemical Technology, College of Engineering, Seoul National University, Seoul 151–742, Korea, KR |
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| Abstract: | Large-scale resolution of epoxides by the yeast Rhodotorula glutinis was demonstrated in an aqueous/organic two-phase cascade membrane bioreactor. Due to the chemical instability and low solubility
of epoxides in aqueous phases, an organic solvent was introduced into the reaction mixture in order to enhance the resolution
of epoxide. A cascade hollow-fiber membrane bioreactor was used (1) to minimize the toxicity of organic solvents towards the
epoxide hydrolase of R. glutinis, and (2) to remove inhibitory amounts of formed diol from the yeast cell containing aqueous phase. Dodecane was selected
as a suitable solvent and 1,2-epoxyhexane as a model substrate. By use of this membrane bioreactor, highly concentrated (0.9 M
in dodecane) enantiopure (> 98% ee) (S)-1,2-epoxyhexane (6.5 g, 30% yield) was obtained from the racemic mixture.
Received: 28 June 1999 / Received revision: 26 August 1999 / Accepted: 3 September 1999 |
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