A new human prostate carcinoma cell line, 22Rv1 |
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| Authors: | R. Michael Sramkoski Thomas G. Pretlow II Joseph M. Giaconia Theresa P. Pretlow Stuart Schwartz Man-Sun Sy Susan R. Marengo Johng S. Rhim Desheng Zhang James W. Jacobberger |
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| Affiliation: | (1) Cancer Research Center, Case Western Reserve University, 10900 Euclid Avenue, 44106-4944 Cleveland, Ohio;(2) Department of Genetics, Case Western Reserve University, 44106 Cleveland, Ohio;(3) Department of Pathology, Case Western Reserve University, 44106 Cleveland, Ohio;(4) Department of Urology, Case Western Reserve University, 44106 Cleveland, Ohio;(5) Laboratory of Biochemical Physiology, National Cancer Institute, 21702 Frederick, Maryland |
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| Abstract: | Summary A cell line has been derived from a human prostatic carcinoma xenograft, CWR22R. This represents one of very few available cell lines representative of this disease. The cell line is derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft. Flow cytometric and cytogenetic analysis showed that this cell line represents one hyper DNA-diploid stem line with two clonal, evolved cytogenetic sublines. The basic karyotype is close to that of the grandparent xenograft, CWR22, and is relatively simple with 50 chromosomes. In nude mice, the line forms tumors with morphology similar to that of the xenografts, and like the parental CWR22 and CWR22R xenografts, this cell line expresses prostate specific antigen. Growth is weakly stimulated by dihydroxytestosterone and lysates are immunoreactive with androgen receptor antibody by Western blot analysis. Growth is stimulated by epidermal growth factor but is not inhibited by transforming growth factor-β1. |
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| Keywords: | cancer epithelial cell xenograft androgen response cell growth EGF TGF-β |
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