Degradation of some phenols and hydroxybenzoates by the imperfect ascomycetous yeastsCandida parapsilosis andArxula adeninivorans: evidence for an operative gentisate pathway |
| |
Authors: | Wouter J. Middelhoven Alex Coenen Bart Kraakman Maarten D. Sollewijn Gelpke |
| |
Affiliation: | (1) Laboratory of Microbiology, Agricultural University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands |
| |
Abstract: | The imperfect ascomycetous yeastsCandida parapsilosis andArxula adeninivorans degraded 3-hydroxybenzoic acid via gentisate which was the cleavage substrate. 4-Hydroxybenzoic acid was metabolized via protocatechuate. No cleavage enzyme for the latter was detected. In stead of this NADH- and NADPH-dependent monooxygenases were present. In cells grown at the expense of hydroquinone and 4-hydroxygenzoic acid, enzymes of the hydroxyhydroquinone variant of the 3-oxoadipate pathway were demonstrated, which also took part in the degradation of 2,4-dihydroxybenzoic acid byC. parapsilosis.Abbreviations HHQ Hydroxyhydroquinone (1,2,4-trihydroxybenzene) - GSH reduced Glutathione |
| |
Keywords: | Arxula adeninivorans Candida parapsilosis gentisate pathway hydroxybenzoate metabolism hydroxyhydroquinone route phenol monooxygenase |
本文献已被 SpringerLink 等数据库收录! |