Isolation,Identification, and Characterization of a Novel,Oil-Degrading Bacterium, <Emphasis Type="Italic">Pseudomonas aeruginosa</Emphasis> T1 |
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Authors: | Mohammad Hasanuzzaman Kathryn M Umadhay-Briones Szilvia M Zsiros Naoki Morita Yoshinobu Nodasaka Isao Yumoto Hidetoshi Okuyama |
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Institution: | (1) Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060-0810, Japan;(2) ROM Co. Ltd., Chuo-ku, Sapporo 064-0804, Japan;(3) National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan;(4) School of Dentistry, Hokkaido University, Kita-ku, Sapporo 060-8586, Japan |
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Abstract: | A novel, oil-degrading bacterium (strain T1) was isolated from a hot spring in Hokkaido, Japan. It efficiently degrades different types of fats and oils, including edible oil waste. When grown in a mineral salt medium containing 1% triacylglycerol (as salad oil), hydrolysis products were 1,3- and 1,2-diacylglycerols, monoacylglycerol, and free fatty acid. However, these products were almost completely consumed during cultivation at 30°C for 5 days, indicating that extracellular lipase acts randomly at different sn-positions of acylglycerols and that strain T1 has a high capacity to utilize free fatty acids. Secreted lipase activity was induced by salad oil and oleic acid. This strain was a Gram-negative straight rod shaped, aerobic, with a polar flagellum, capable of growing in temperature ranges between 15°C and 55°C. The 16S rRNA gene sequence analysis and DNA-DNA hybridization revealed it as a new strain of Pseudomonas aeruginosa. The type strain was T1. |
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