Effects of various agents on DNA fragmentation and telomerase enzyme activities in adenocarcinoma cell lines |
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Authors: | Didem Turgut Cosan Ahu Soyocak Ayse Basaran ?rfan Degirmenci Hasan Veysi Gunes Fezan Mutlu Sahin |
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Institution: | 1.Eski?ehir Osmangazi üniversitesi,Eski?ehir,Türkiye;2.Eski?ehir Osmangazi üniversitesi,Eski?ehir,Türkiye |
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Abstract: | Natural compounds such as resveratrol, tannic acid, and quercetin may help to treat cancer. Tamoxifen is a non-steroidal anti-estrogen
drug widely used in the treatment of patients with estrogen receptor-positive breast cancer. The aim of the study was to compare
the effects of these natural compounds and tamoxifen in colon adenocarcinoma (CaCo-2) and breast adenocarcinoma (MCF-7) cell
lines, on telomerase enzyme activity, cell viability, number of cells and DNA fragmentation. In this study to determine telomerase
enzyme activity was used PCR–ELISA kit. To determine cell viability and number of cells were used tripan blue stain. DNA fragmentation
was determined by DNA ladder isolation kit. Tannic acid was more effective than resveratrol, with respect to reduction in
telomerase activity, cell viability and cell count in breast adenocarcinoma. Tannic acid and tamoxifen was more effective
than resveratrol and quercetin telomerase activity, cell viability and cell count in colon adenocarcinoma. Flavonoids such
as resveratrol, tannic acid and quercetin which was studied on, has benefical effects on cancer therapy. These effects such
as decreasing telomerase enzyme activity, cell viability and number of cells and inducing DNA fragmentation (apoptosis) must
be studied for assist to develop new therapeutic pathways. There should be much more sudies in order to discover resveratrol,
tannic acid and quercetin and other potential medicines. |
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