A systemic proteomic analysis of <Emphasis Type="Italic">Populus</Emphasis> chloroplast by using shotgun method |
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Authors: | Hong-Mei?Yuan Kai-Long?Li Rui-Juan?Ni Wen-Dong?Guo Zhuo?Shen Chuan-Ping?Yang Bai-Chen?Wang Gui-Feng?Liu Chang-Hong?Guo Email author" target="_blank">Jing?JiangEmail author |
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Institution: | (1) Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China;(2) College of Life Science, Daqing Normal University, Xibinxi Road, Daqing, 163712, China;(3) Institute of Natural Resources and Ecology, Heilongjiang Academy of Sciences, 103 Haping Road, Harbin, 150040, China;(4) College of Life Science and Biotechnology, Harbin Normal University, 50 Hexing Road, Harbin, 150080, China |
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Abstract: | The chloroplast is one of the most important organelles in plants. Proteomic investigations of chloroplasts have been undertaken
for many herb plant species, but to date no such investigation has been reported for woody plant chloroplasts. In the present
study we initiated a systematic proteomic study of Populus chloroplasts using a shotgun proteomic method. After isolation of chloroplasts and tryptic digestion of the proteins, the
protein fragments were separated via HPLC using an SCX column, and the peptides were analyzed by LC-MS/MS; 119 proteins were
successfully identified. Based on annotation information in the UniProtKB/Swiss-Prot database, these proteins were identified
as being localized in the chloroplast thylakoid membrane, chloroplast stroma, chloroplast thylakoid lumen, and plastoglobules.
Over 50% of all identified proteins were confirmed as chloroplast thylakoid proteins, and 85 are encoded by the chloroplast
genome with the remaining proteins encoded by the nuclear genome. Based on functional annotation, these proteins were classified
into four functional categories, including photosynthesis, redox regulation and stress, primary and secondary metabolism,
transport and signaling. These data provide a valuable basis for further studies on photosynthesis in poplar species. |
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