5-hydroxytryptamine synthesis in HL-1 cells and neonatal rat cardiocytes |
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| Authors: | Ikeda Keiichi Tojo Katsuyoshi Otsubo Chikara Udagawa Takashi Kumazawa Kensuke Ishikawa Masahiro Tokudome Goro Hosoya Tatsuo Tajima Naoko Claycomb William C Nakao Kazuwa Kawamura Masahiro |
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| Affiliation: | Department of Pharmacology I, Jikei University School of Medicine, Minato-ku, Tokyo 105-8461, Japan. ikedak@jikei.ac.jp |
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| Abstract: | Some reports showed that serotonergic system might have existed and that 5-hydroxytryptamine (5-HT) was detected in the hamster heart. The source of 5-HT in the heart, however, remains to be fully elucidated. So the present study was designed to define serotonergic system and to clarify which cell could produce 5-HT in the heart. As a result, 5-HT was detected in homogenates of HL-1 cardiomyocytes by high performance liquid chromatography with fluorescence detection, but not in those of neonatal rat non-cardiomyocytes (NMCs). And TPH and AADC mRNAs were expressed in HL-1 cardiomyocytes and neonatal rat cardiomyocytes (MCs), not in NMCs. mRNAs of 5-HT(2A) receptor were detected in both MCs and NMCs, and those of 5-HT(2B) receptor in NMCs. These findings definitively demonstrate that 5-HT is secreted from the myocytes of the heart and strongly implied that 5-HT might play a certain role in cardiac physiology. |
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| Keywords: | 5-Hydroxytryptamine HL-1 cardiomyocytes Neonatal rat cardiac myocytes Neonatal rat cardiac non-myocytes HLPC |
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