Germline recombination in a novel Cre transgenic line,Prl3b1‐Cre mouse |
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| Authors: | Al‐Sayed Al‐Soudy Tsuyoshi Nakanishi Seiya Mizuno Yoshikazu Hasegawa Hossam H Shawki Megumi C Katoh Walaa A Basha Abdelaziz E Ibrahim Hany A El‐Shemy Hiroyoshi Iseki Atsushi Yoshiki Youhei Hiromori Hisamitsu Nagase Satoru Takahashi Hisashi Oishi Fumihiro Sugiyama |
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| Institution: | 1. Laboratory Animal Resource Center, University of Tsukuba, Tsukuba, Ibaraki, Japan;2. Animal Genetic Resource Department, National Gene Bank, Giza, Egypt;3. Department of Anatomy and Embryology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan;4. Laboratory of Hygienic Chemistry and Molecular Toxicology, Gifu Pharmaceutical University, Gifu, Japan;5. Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt;6. Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt;7. Cairo University Research Park, Faculty of Agriculture, Cairo University, Giza, Egypt;8. International Institute for Integrative Sleep Medicine (WPI‐IIIS), University of Tsukuba, Tsukuba, Ibaraki, Japan;9. Experimental Animal Division, Riken BioResource Center, Tsukuba, Japan;10. Department of Pharmacy, College of Pharmacy, Kinjo Gakuin University, Aichi, Japan |
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| Abstract: | Spermatogenesis is a complex and highly regulated process by which spermatogonial stem cells differentiate into spermatozoa. To better understand the molecular mechanisms of the process, the Cre/loxP system has been widely utilized for conditional gene knockout in mice. In this study, we generated a transgenic mouse line that expresses Cre recombinase under the control of the 2.5 kbp of the Prolactin family 3, subfamily b, member 1 (Prl3b1) gene promoter (Prl3b1‐cre). Prl3b1 was initially reported to code for placental lactogen 2 (PL‐2) protein in placenta along with increased expression toward the end of pregnancy. PL‐2 was found to be expressed in germ cells in the testis, especially in spermatocytes. To analyze the specificity and efficiency of Cre recombinase activity in Prl3b1‐cre mice, the mice were mated with reporter R26GRR mice, which express GFP ubiquitously before and tdsRed exclusively after Cre recombination. The systemic examination of Prl3b1‐cre;R26GRR mice revealed that tdsRed‐positive cells were detected only in the testis and epididymis. Fluorescence imaging of Prl3b1‐cre;R26GRR testes suggested that Cre‐mediated recombination took place in the germ cells with approximately 74% efficiency determined by in vitro fertilization. In conclusion, our results suggest that the Prl3b1‐cre mice line provides a unique resource to understand testicular germ‐cell development. genesis 54:389–397, 2016. © 2016 Wiley Periodicals, Inc. |
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| Keywords: | Prl3b1 placental lactogen testis spermatogenesis Cre/loxP mouse |
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