Enhanced alpha - amylase production by chromosomal integration of pTVA1 in industrial strain in Bacillus subtilis |
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| Authors: | M. Slugeňová G. Bukovská J. Turňa J. Timko |
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| Affiliation: | (1) Institute of Molecular Biology, Slovak Academy of Science, Dúbravská cesta 21, 842 51 Bratislava, Slovak Republic;(2) Department of Molecular Biology, Comenius University, Bratislava, Slovak Republic |
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| Abstract: | Summary Strain Bacillus subtilis MS was constructed with 12–22 fold increase of  -amylase production, caused by presence of multiple -amylase gene copies in the chromosome of industrial strain Bacillus subtilis CCM2722, as demonstrated by DNA hybridization. The enhanced production is a result of multiple integration of plasmid pTVA1, carrying a temperature sensitive origin of replication from pE194, and containing the -amylase gene and a modified transposon Tn917. |
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