<Emphasis Type="Italic">In vitro</Emphasis> propagation of ten threatened species of <Emphasis Type="Italic">Mammillaria</Emphasis> (Cactaceae) |
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Authors: | R Ramirez-Malagon I Aguilar-Ramirez A Borodanenko L Perez-Moreno J L Barrera-Guerra H G Nuñez-Palenius N Ochoa-Alejo |
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Institution: | (1) Instituto de Ciencias Agrícolas, Universidad de Guanajuato, AP 311, Irapuato, Guanajuato, 36500, Mexico;(2) Departamento de Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados del I.P.N. (Cinvestav) Campus Guanajuato, Km 9.6 Libramiento Norte Carretera Irapuato-León, Irapuato, Guanajuato, 36500, Mexico |
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Abstract: | Mammillaria species are the most numerous within Cactaceae family, and some of them are threatened with extinction as a result of human
activities. In this work, results of in vitro propagation are presented for ten Mammillaria species, testing 20 combinations of indole-3-acetic acid (IAA) and kinetin. Best results on shoot formation were obtained
using kinetin at two levels: 27.9 and 46.5 μM. All IAA levels tested were able to induce de novo shoot formation in M. bocasana, M. densispina, M. hahniana, M. hutchisoniana, M. orcutii, M. pectinifera, M. perbella, M. picta, M. rhodantha, and M. zephyranthoides. Depending on the IAA level tested, four responding groups were observed concerning their highest shoot-formation number.
For all species, the highest average of shoot formation was achieved with 5.7:46.5 or 11.4:46.5 μM IAA/kinetin, yielding 4.8
and 4.7 shoots per explant, respectively, in 60 d. Rooting of regenerated shoots was achieved by leaving the explants in their
shoot-induction medium or transferring them to half-strength MS medium. Hardening of regenerated plants was successfully achieved
by planting them in peat moss substrate after a desiccation treatment at room temperature for 3 d. |
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Keywords: | Cacti Ornamentals Endangered species Plant growth regulators In vitro regeneration |
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