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Keratinase Production by Newly Isolated Antarctic Actinomycete Strains |
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| Authors: | Adriana Gushterova Evgenia Vasileva-Tonkova Elitza Dimova Peter Nedkov Thomas Haertlé |
| Affiliation: | (1) Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev str., bl. 26, 1113 Sofia, Bulgaria;(2) Institute of Organic Chemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev str., bl. 9, Sofia, 1113, Bulgaria;(3) Laboratorie d’Etude des Interactions des Molecules Alimentaires, Institut National de la Recherche Agronomique, Nantes Cedex 3, 44316, France |
| Abstract: | Summary The ability of actinomycete strains newly isolated from Antarctic soils to produce keratinolytic enzymes during growth on sheep wool waste was investigated. The strains which displayed highest keratinase activity and identified as Streptomyces flavis 2BG (mesophilic) and Microbispora aerata IMBAS-11A (thermophilic) were selected for a more detailed analysis. The addition of starch to the growth medium affected keratinase secretion by both strains. After 5 days of cultivation, a 6-fold increase in keratinase activity of strain 11A was observed in the presence of 11 g starch/l and a 9-fold increase in keratinase activity of the strain 2BG in the presence of 5 g starch/l. The results obtained showed that both newly isolated strains are very promising for effective processing of native keratinous wastes. To our knowledge, this is the first report of Antarctic actinomycete strains that were able to grow on keratin-containing wastes by producing keratinolytic enzymes. |
| Keywords: | Antarctic actinomycetes keratinase activity Microbispora aerata Streptomyces flavis wool waste |
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