Characterization of Aeromonas salmonicida variants with altered cell surfaces and their use in studying surface protein assembly |
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Authors: | Steven G Griffiths William H Lynch |
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Institution: | (1) Department of Biology, University of New Brunswick, E3B 6E1 Fredericton, N.B., Canada |
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Abstract: | Aeromonas salmonicida variants were characterized for alterations in their cell surface structure and used to examine reconstitution of the surface protein layer (A-layer). Variants lacking outer membrane O-polysaccharide were devoid of A-layer and excreted stainable floret-like material of the surface protein (A-protein). One variant, showing partial loss of O-polysaccharide, was associated with a disrupted A-layer and excretion of some A-protein. Variants lacking A-protein but possessing O-polysaccharide rapidly absorbed and concentrated sufficient excreted A-protein at the cell surface to coat the cells with a single confluent layer. Although differences in electrophoretic mobilities of A-proteins and O-polysaccharides from typical and atypical strains were evident, the different A-proteins and A-protein-deficient variants were interchangeable for reconstitution of a surface protein layer. No association of A-protein with cell surfaces of unrelated gram-negative bacteria was observed.Abbreviations A-layer
additional surface protein layer
- A-protein
surface protein
- Ast
Aeromonas salmonicida typical
- Asa
Aeromonas salmonicida atypical
- A-
phenotypically A-protein-negative variant
- O-
phenotypically O-polysaccharide-negative variant
- Owk
phenotypically O-polysaccharide weak variant
- BHI
brain heart infusion
- SDS-PAGE
sodium dodecylsulfate-polyacrylamide gel electrophoresis
- TEM
transmission electron microscopy |
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Keywords: | typicalgif" alt="ldquo" align="MIDDLE" BORDER="0">typical and gif" alt="rdquo" align="MIDDLE" BORDER="0"> and atypicalgif" alt="ldquo" align="MIDDLE" BORDER="0">atypical Aeromonas salmonicida variants" target="_blank">gif" alt="rdquo" align="MIDDLE" BORDER="0"> Aeromonas salmonicida variants A-layer O-polysaccharide A-protein reconstitution |
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