Toxic effects of titanium dioxide nanoparticles on microbial activity and metabolic flux |
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| Authors: | Soohyun Park Soojin Lee Borim Kim Sunhee Lee Juhee Lee Sangjun Sim Manbock Gu Jongheop Yi Jinwon Lee |
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| Institution: | 1.Department of Chemical and Biomolecular Engineering,Sogang University,Seoul,Korea;2.Department of Chemical Engineering,Sungkyunkwan University,Suwon,Korea;3.College of Life Sciences and Biotechnology,Korea University,Seoul,Korea;4.School of Chemical and Biological Engineering,Seoul National University,Seoul,Korea |
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| Abstract: | The purpose of this research is to estimate and quantify the toxicity of titanium dioxide (TiO2) nanoparticles in microorganisms. Nano-sized particles of TiO2 were more toxic compared to micro-sized particles. Three microorganismal species, Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae, were used to test TiO2 antimicrobial effects. E. coli showed the lowest survival rate (36%), while S. cerevisiae showed the highest survival rate (71%). The antimicrobial effect of TiO2 was also dependent on ultraviolet ray wavelength. The survival ratio of E. coli was 40% at a 254 nm wavelength and 80% at 365 nm. To observe the effect of TiO2 on the intracellular metabolism, a metabolic flux analysis and the measurement of in vivo glucose-6-phosphate were performed.
G6P concentration in cells exposed to TiO2 increased, and glycolysis flux was also higher than the controls. |
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