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Apical surface of the epithelial cells in the gallbladder of the rainbow trout and the tench
Authors:G. Viehberger
Affiliation:(1) Department of Natural Science, Medgar Evers College, Cuny and The East Coast Neuroscience, Foundation, Inc., USA;(2) Department of Natural Sciences, Medgar Evers College, 1150 Carroll Street, 11225 Brooklyn, New York, USA
Abstract:Summary The aluminium-formaldehyde (ALFA) histofluorescence method was used to study the innervation of the gill of the marine bivalve mollusc Mytilus edulis and the results were contrasted with those obtained with the standard formaldehyde-induced-fluorescence (FIF) method. The ALFA method produced more fluorescing structures than the FIF method, thus revealing fine branches of the branchial nerve running beneath the gill epithelium which previously remained undetected. This study demonstrates the usefulness of the ALFA histofluorescence method in the study of marine invertebrates.This study was supported in part by Grants 1506RR08171 from NIMH and 5T32GM07641 from the MARC Program of NIGMS. I wish to thank E. Aiello for thoughtful discussions of the work
Keywords:Histoflourescence  Monoamines  Mytilus edulis  Gill innervation
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