Effects of Gamma-Irradiation on the Activity of Tonoplast H+-ATPase from Potato Tubers (Solanum tuberosum L.) |
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Authors: | Todoriki Setsuko; Hayashi Toru; Nakamura Yoshiyuki; Kasamo Kunihiro |
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Institution: | National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries 2-1-2 Kannondai, Tsukuba, Ibaraki, 305 Japan |
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Abstract: | Tonoplast vesicles were prepared from potato tubers (Solariumtuberosum L.) on a step gradient (0% and 6%, w/w) of dextranT-70 to clarify the mechanism by which the tonoplast H+-ATPaseis inactivated by gamma-irradiation. H+-ATPase activity andH+ -pumping were examined after irradiation of tubers (in vivoirradiation) and of isolated tonoplast vesicles (in vitro irradiation)at doses up to 1.0 kGy. Both in vivo irradiation and in vitroirradiation resulted in significant decreases in ATPase andH+-pumping activities. The ATPase and H+-pumping activities12 h after irradiation were much lower than those 2 h afterirradiation. Solubilized H+-ATPase was inactivated, in a dose-dependentmanner, by irradiation (enzyme irradiation) to a greater extentthan was observed after in vitro irradiation or in vivo irradiation.The activity of ATPase 12 h after enzyme irradiation was almostthe same as it was 2 h after enzyme irradiation. The free fattyacid content of vacuolar membranes was increased by in vivoirradiation and by in vitro irradiation with an accompanyingdecrease in tonoplast H+-ATPase activity. Lipids from irradiatedtonoplasts had a considerable inhibitory effect on the activityof solubilized H+-ATPase. This result suggests that the directinactivation of H+-ATPase in potato tonoplast by gamma-irradiationis augmented by the effects of deterioration of membrane lipidsthat is induced by the irradiation. (Received December 21, 1994; Accepted May 16, 1994) |
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