The distribution of 4-hydroxynonenal-modified proteins in gastric mucosa of duodenal peptic ulcer patients |
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| Authors: | Yelisyeyeva Olha Cherkas Andriy Zarkovic Kamelija Semen Khrystyna Kaminskyy Danylo Waeg Georg Zarkovic Neven |
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| Institution: |
a Department of Histology, Cytology & Embryology, Lviv, Ukraine
b Department of Hospital Therapy, Lviv National Medical University, Lviv, Ukraine
c Emergency Care Department, Novoyavorivsk Regional Hospital No.1, Novoyavorivsk, Ukraine
d Division of Pathology, Clinical Hospital Centre & Medical Faculty, Zagreb, Croatia
e Department of Pharmaceutical Chemistry, Lviv National Medical University, Lviv, Ukraine
f Institute of Molecular Biosciences, Karl Franz University, Graz, Austria
g Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia |
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| Abstract: | This study used monoclonal antibody specific for 4-hydroxynonenal (HNE)-histidine to evaluate immunohistochemical distribution of HNE-protein adducts in gastric mucosa biopsies of 52 peptic ulcer patients (all positive for H. pylori) and of 20 healthy volunteers (eight positive and 12 negative for H. pylori). HNE-modified proteins were present in glandular epithelium in all subjects, both patients with duodenal peptic ulcer and healthy subjects. Hence, the presence of HNE did not appear to be related to the presence of H. pylori. However, in patients with duodenal peptic ulcer accumulation of HNE-protein adducts was frequently observed also in nuclei, while in the control group such subcellular distribution of HNE was not observed at all. This study shows physiological presence of HNE in human gastric mucosa, but also suggests its role in pathology of gastric dysfunction in duodenal peptic ulcer patients manifested by accumulation of HNE-protein adducts in particular in nuclei of gastric glandular epithelium. |
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| Keywords: | Lipid peroxidation 4-hydroxynonenal oxidative stress Helicobacter pylori peptic ulcer gastric mucosa |
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