Rapid Identification of δ-endotoxin from Bacillus thuringiensis subsp. kurstaki HD-1 with Proteomic Analysis |
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| Institution: | 1. Ilsong Institute of Life Science, Hallym University, Anyang 431-060, Republic of Korea;2. School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea;1. Dept. of Earth Sciences, Science Laboratories, South Road, Durham DH1 3LE, UK;2. Dept. of Civil Engineering, University of Bristol, Queen’s Building, University Walk, Bristol BS8 1TR, UK;3. Dept. of Geography, Science Laboratories, South Road, Durham DH1 3LE, UK;1. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan;2. Japan Society for the Promotion of Science, Sakyo-ku, Kyoto 606-8502, Japan;3. Graduate School of Advanced Integrated Studies in Human Survivability, Kyoto University, Sakyo-ku, Kyoto 606-8306, Japan;1. Renal Department, UHCW NHS Trust, Coventry, UK;2. Renal Department, St. James’s University Hospital, Leeds, UK;3. School of Engineering, University of Warwick, Coventry, UK;4. H&I department, NHSBT, Birmingham, UK;5. H&I department, Royal Liverpool University Hospital, Liverpool, UK;6. University of Oklahoma Health Science Center, Oklahoma City, OK, United States;7. Pure Protein LLC, Oklahoma City, OK, United States;8. Immunohaematology and Blood Transfusion, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands;9. Warwick Medical School, University of Warwick, Coventry, UK;1. Department of Industrial Management, National Taiwan University of Science and Technology, No. 43, Section 4, Kee-Lung Road, Taipei 106, Taiwan;2. HIWIN Technologies Corp., No. 7, Jingke Road, Taichung Precision Machinery Park, Taichung 408, Taiwan;3. Department of Urology, Taipei Veterans General Hospital, Institute of Clinical Medicine and Department of Urology, School of Medicine, National Yang-Ming University, 201, Section 2, Shih-Pai Road, Taipei 112, Taiwan;4. Superintendent Office, Taipei Veterans General Hospital, 201, Section 2, Shih-Pai Road, Taipei 112, Taiwan;1. Department of Clinical Radiology, Oxford University Hospitals NHS Trust, Churchill Hospital, Old Road, Headington, Oxford, Oxfordshire OX3 7LE, UK;2. Department of Oncology, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Headington, Oxford, Oxfordshire OX3 7DQ,UK;3. Radiation Physics and Protection, Oxford University Hospitals NHS Trust, Churchill Hospital, Old Road, Headington, Oxford, Oxfordshire OX3 7LE, UK |
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| Abstract: | This study was carried out to identify rapidly δ-endotoxin from Bacillus thuringiensis (Bt) subsp. kurstaki HD-1 with proteomic analysis. Protoxin was isolated from sporulated cells and purified by ultracen-trifugation using 40-70% sucrose density gradient. Protoxin was treated with trypsin to analyze digested peptides by liquid chromatographytandem mass spectrometry. The proteomic analysis for detected peptides revealed that this methodology is available for discriminating similar Bt strains by identifying Bt subsp. kurstaki HD-1-specific peptides, suggesting that proteomic analysis can be used for rapid identification of Bt strains. |
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