Cost reduction in the micropropagation of banana by using tubular skylights as source for natural lighting |
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Authors: | Andrea Kodym Stefan Hollenthoner Francisco Javier Zapata-Arias |
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Institution: | (1) Plant Breeding Unit, FAO/IAEA Agriculture & Biotechnology Laboratory, Agency's Laboratories, A-2444 Seibersdorf, Austria;(2) Instrumentation Unit, Physics-Chemistry-Instrumentation Laboratory, Agency's Laboratories, A-2444 Seibersdorf, Austria |
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Abstract: | Summary Daylight instead of artificial light was exploited for the in vitro culture of banana. Tubular skylights rediverted natural light into an interior enelosed room whereby each skylight, available
for ca. US$600, could sufficiently illuminate an area of 3–5 m2. The maintenance-free system allowed only a minimum of heat transfer and no cooling was necessary. The culture room required
no electricity supply and under our conditions savings on costs for electricity of US$6 m−2 wk−1 were achieved, as compared to a standard growth room equipped with artificial lighting and controlled photoperiod and temperature
regimes. Under natural light conditions, micropropagated plantlets were well developed at mean photosynthetic photon flux
densities (PPFD) of 5–13 μmol m−2 s−1 and photoperiods of 9–14 h. Micropropagation rates were either the same or significantly higher than under artificial lighting.
Single shoots on rooting medium showed some symptoms of etiolation, yet acclimatization rates averaged 95%. A step-like culture
rack. rather than a vertical one, permitted uniform plant growth on all levels. This paper describes an established micropropagation
system of low cost and simplicity. |
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Keywords: | daylight growth room in vitro culture Musa Solatube sunlight tissue culture |
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